Multiple choice

Which among the following is a nuclease enzyme?

  1. Hind III

  2. Maltase

  3. Pepsin

  4. Trypsin

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A Correct answer
Explanation

Hind III is a type II site-specific deoxyribonuclease restriction enzyme isolated from Haemophilus influenzae that cleaves the palindromic DNA sequence AAGCTT in the presence of the cofactor Mg2+ via hydrolysis. The cleavage of this sequence between the AA's results in 5' overhangs on the DNA called sticky ends.