Multiple choice

Proteins are the building blocks of life. All the major processes in body are regulated by one or the other protein. Therefore, it becomes very vital to assess proteins in a professional manner, for which many sophisticated techniques and protocols are available. The standard protein assessment protocol includes: Purification, Digestion, Sequencing, Assays. Based on the provided information and knowledge of protein assessment methods, answer the following question:

Which of the following would you use before the sequencing of a peptide?

  1. Dansylation

  2. Microarray protein expression

  3. Enzymatic fragmentation

  4. Fast Protein Liquid Chromatography
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C Correct answer
Explanation

Before sequencing of peptides using mass spectrometry and after purification of peptides from the mixture, peptides need to be cleaved by different enzymes in order to have standardized sequencing. 

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