Multiple choice

Which technique is used to introduce genes into dicots?

  1. Electroporation

  2. Particle acceleration

  3. Microinjection

  4. Ti plasmid infection
Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

Ti plasmid from Agrobacterium tumefaciens is the preferred method for introducing genes into dicot plants because Agrobacterium naturally infects dicots. The T-DNA region of the Ti plasmid integrates into the plant genome. Electroporation, particle acceleration (gene gun), and microinjection are physical methods typically used for monocots which are recalcitrant to Agrobacterium infection.

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