Multiple choice

Which step of DNA sequencing is skipped during shot-gun sequencing?

  1. Computer analysis

  2. Cloning of DNA fragment

  3. Primer reaction

  4. Mapping step
Reveal answer Fill a bubble to check yourself
D Correct answer
Explanation

Shotgun sequencing randomly fragments the entire genome and sequences the fragments, then uses computational methods to assemble them. Unlike hierarchical (clone-by-clone) sequencing, it skips the labor-intensive step of creating a detailed physical map of the genome before sequencing.

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