Multiple choice Why is heat used to sterilize nonliving objects in tissue culture? Proteins are denatured at temperature above 55oC. Proteins lose their tertiary structures due to break down of bonds. Both are correct. Proteins synthesis does not occur at high temperature. Both are incorrect. Reveal answer Fill a bubble to check yourself C Correct answer Explanation Heat is used to sterilize nonliving objects in tissue culture as the proteins are natured and their tertiary structures are lost at high temperature.