Multiple choice

In which of the following stages of genetic engineering is a probe used?

  1. Cutting the target DNA

  2. Cloning rDNA

  3. Screening for recombinants

  4. Recombination of target DNA and vector

  5. Insertion of rDNA in a host cell
Reveal answer Fill a bubble to check yourself
C Correct answer
Explanation

To know which host cells have taken up the recombined DNA, a radioactive nucleic acid probe, complementary to the gene of interest is added to the growth media. It enters the cells and if a cell contains the gene of interest, it binds to that region of DNA. The colony that emits radioactivity indicates the presence of the gene.

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