Multiple choice

Which of the following PCR formats is/are used in the detection of both DNA and RNA viruses?

P. RT-PCR Q. IC-PCR R. IC-RT-PCR

  1. Only P

  2. Only Q

  3. Only R

  4. P and Q

  5. Q and R
Reveal answer Fill a bubble to check yourself
E Correct answer
Explanation

Correct answer. Nucleic acid based detection systems were greatly improved following the development of the polymerase improved following the development of the polymerase chain reaction (PCR). PCR is able to detect pathogens by targeting their genetic material (RNA/DNA). Real time PCR (RT-PCR) is used to combine amplification, detection and quantification in a single step for RNA as target. Immunocapture PCR (IC-PCR) is used to combine the advantages of serology and is a very sensitive method of detection the diseases by targeting both DNA and RNA as genetic material. Multiplex PCR (IC-RT-PCR) allows for simultaneous and sensitive detection of different DNA and RNA as genetic material in the detection of plant diseases in a single reaction.

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