Multiple choice

Which one of the following dyes is used in testing the viability of protoplasts?

  1. Evans blue

  2. Trypan blue

  3. Fluroscein diacetate

  4. Phenosafranin
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A Correct answer
Explanation

Evans blue is a vital stain that cannot penetrate live protoplast membranes. Viable protoplasts exclude the dye and remain unstained, while dead/damaged protoplasts take up the blue color. Trypan blue has similar use but Evans blue is more commonly used for protoplasts specifically. Fluorescein diacetate stains viable cells green.

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