Multiple choice

Dimethyl sulfoxide (DMSO) is used as a cryopreservant for mammalian cell cultures because

  1. it is an organic solvent

  2. it easily penetrates cells

  3. it protects cells by preventing crystallisation of water

  4. it is also utilised as a nutrient
Reveal answer Fill a bubble to check yourself
C Correct answer
Explanation

DMSO (dimethyl sulfoxide) is a cryoprotectant that penetrates cell membranes and interacts with water molecules. By lowering the freezing point and interfering with ice crystal formation, it prevents cellular damage during freezing. This protection is essential for maintaining cell viability in cryopreservation. The other options are incorrect: being an organic solvent (A) is irrelevant, cell penetration (B) alone doesn't explain protection, and DMSO is not a nutrient (D).

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