Multiple choice Mitral (bicuspid) valve in mammals guard the opening between left auricle and left-ventricle pulmonary vein and left auricle stomach and intestine liver and spleen Reveal answer Fill a bubble to check yourself A Correct answer Explanation The mitral (bicuspid) valve is located between the left atrium (auricle) and left ventricle of the heart. It prevents backflow of blood from the left ventricle to the left atrium when the ventricle contracts, ensuring one-way flow toward the aorta.