Multiple choice

A flask containing 100 ml and a bioreactor containing 1 liter of nutrient broth were inoculated with 500 cells of a certain bacteria sp. The flask was incubated on mechanical shaker and aeration was introduced in bioreactor at 37°C for 2 hrs. The final pH in flask was found to be 5 with 105 cells/ml and that in bioreactor was 7 with 1012 cell/ml.

Calculate the generation time of that bacteria sp. In flask.

  1. 158 min

  2. 15.80 min

  3. 3.90 min

  4. 39 min

Reveal answer Fill a bubble to check yourself
A Correct answer
Explanation

15.80 min: Generation time (G) = total time (t)/{3.3 log (final number of cells)/(initial number of cells)}.