Multiple choice

A scientist produced an enzyme by microbial fermentation. He wanted to test it and thus, disintegrated it into its constituent parts: apoenzyme and cofactor. Each of the parts is heated for 3 minutes at 100°C. Subsequently, various combinations are tested for their ability to perform the enzymatic function. Which of the following combinations will retain its functionality?

  1. Unheated apoenzyme and unheated cofactor.

  2. Heated apoenzyme and unheated cofactor.

  3. Heated apoenzyme and heated cofactor.

  4. More than one of the above combinations is possible.
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A Correct answer
Explanation

Unheated apoenzyme and unheated cofactor. An enzyme is made up of two parts- apoenzyme, that is the protein part and a cofactor that is the non protein part. Since apoenzyme is protein in nature, if it is heated at a high temperature, it will get denatured. But since cofactor is non protein in nature, it remains unaltered even at high temperature.

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