Multiple choice

If a person obtains transformants by inserting a recombinant DNA within the coding sequence of â-galactosidase, he or she will separate out recombinants from non-recombinants by observing

  1. non-recombinant colonies do not produce any colour, whereas recombinants form blue coloured colonies

  2. recombinant colonies do not produce any colour, whereas non-recombinants form blue coloured colonies

  3. recombinants and non-recombinants both produce blue coloured colonies

  4. no colonies are formed due to insertional inactivation

Reveal answer Fill a bubble to check yourself
B Correct answer
Explanation

When a person obtains transformants by inserting a recombinant DNA within the coding sequence of â-galactosidase, the recombinant colonies do not produce any colour, whereas non-recombinants form blue coloured colonies. This observation helps in separating out the recombinants from the non-recombinant ones.