An ASP.NET application can have only one Global.asax file per application or virtual directory. The Global.asax file contains application-level event handlers, and having multiple files would cause conflicts. The single file handles all application and session events.
ASP.NET applications support only a single Global.asax file per application, which handles application-level events like Application_Start and Session_Start; having more than one (or even exactly two) is not supported by the framework, so the statement is false.